Pacific blue fluorophore.

Fluorophore type. Organic dye. Organic dye. Organic dye (tandem), resonance energy transfer from PE molecule to eFluor 610 dye. Organic dye (tandem), resonance energy transfer from PerCP molecule to eFluor 710 dye. Ex (nm) 475. 546. 496, 565. 488. Em (nm) 535. 585. 607. 710. Application. ICC, IHC. ICC, IHC. FC. FC. Conjugates available : eFluor ... Texas Red (and Texas Red-X) Dye. Invitrogen Texas Red dye is a bright red-fluorescent dye with excitation ideally suited to the 561 or 594 nm laser lines. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Texas Red sulfonyl chloride is susceptible to hydrolysis and its low solubility in water may complicate ...Fluorescent probes or fluorophores (fluorescent dyes or proteins) are coupled to a secondary antibody or streptavidin to allow visualization of an analyte. Each fluorophore has its own spectral characteristics, with excitation and emission spectra particular to the molecule. Use the spectra viewer to build dye panels and compare the suitability ...Pacific Green dye is a bright green-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 411/510 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 ...

NovaFluor Blue 510 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. With distinct spectral signatures, NovaFluor Blue 510, NovaFluor Blue 530, and NovaFluor Blue 555 dyes can be used together in a flow cytometry panel. The macromolecule-based NovaFluor Blue 510 dye ...* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.f.hubspotusercontent00.net

Pacific Blue 455 V4. BV480. 480 BV510 510. eFluor506. 510. V500. 510 V8 Pacific Orange 550 V9. BV570. 570. Super Bright 600. 600 BV605 605 V11 BV650 650. Super Bright 702. 702 BV711 711 V14. BV750. 750 ... 830nm) of eac h fluorochrome providing a unique signature for each dye, this allows combinations that conventional cytometers cannot us …Hypoxyprobe Pacific Blue Kit (100 mg pimonidazole HCl plus 1 unit of Rat IgG1 monoclonal antibody conjugated to Pacific Blue fluorophore (HP-Pacific Blue)) Item# hp15-100kit $459.00

Fluorochrome chart FL 450/50 530/30 585/40 630/30 670/40 720/40 780/60 RB Waeeth 3 400 450 500 550 600 650 700 750 800 850 R-PE-Cy7 Cy7 APC-Cy7 AF700 APC-Cy5.5 er DRAQ5 er AF647 R-PE-Cy5.5 R-PE-Cy5 Cy5 APC 7-AAD TR AF594 R-PE-TR PI AF568 R-PE AF555 Cy3 FITC AF488 GFP PB BV421 AF405 DAPI ee uorescet chae n lue chnnel …After staining a cell population with Pacific Blue™, annexin V and SYTOX™ AADvanced™, apoptotic cells show violet fluorescence, dead cells show red fluorescence, and live cells show little or no fluorescence. These populations are easily distinguished by a flow cytometer with the 405 nm and 488 nm lines for excitation.Learn about the features and applications of CF® dyes, Biotium's next generation fluorescent dyes for biological research. Download the CF® dye brochure and explore the wide range of colors and conjugates available.Pacific Orange and Pacific Blue antibody labeling kits enable attachment of a violet light-excitable fluorescent label on antibodies (up to 100 µg per reaction). Each kit contains enough dye for five reactions. Product Overview Documents Showing 2 of 2 Save to list Add to cart Recurring order eligibleNovember 5, 2020. Source: Technische Universität Dresden. Summary: Blue phosphorus, an atomically thin synthetic semiconductor, becomes metallic as soon as it is converted into a double layer ...

Note that the standard Dapi/Hoechst filter set will not work with fluorophores that excite at 405 nm. For instance, Alexa 405 and Pacific Blue are bad choices for widefield scopes with standard Dapi/Hoechst filter sets that excite at 365 nm.

We have put together a comprehensive list of fluorochromes compatible with our MACSQuant Instruments. Multi-color flow cytometry and cell sorting applications require the use of a wide range of fluorochrome-conjugated antibodies and dyes. MACSQuant X, MACSQuant Analyzer 10, and MACSQuant Tyto® compatible dyes. Laser.

In today’s fast-paced world, time is of the essence. With busy schedules and limited time, it is essential to find ways to streamline our tasks and make things more convenient. This applies not only to our daily activities but also to our t...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. …Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.405 Blue Brilliant Violet ™ 421 BD Horizon™ V450 VPD450 Pacific Blue™ Green BD Horizon V500 AmCyan Orange Brilliant Violet ™ 605a 375b Blue Hoechst 33342 BD Influx™ 488 Green FITC Alexa Fluor® 488 Yellow PE PI Orange BD Horizon PE-CF594 PE-Texas Red® Red 7-AAD PE-Cy5 PerCP PerCP-Cy5.5 Infrared PE-Cy7 532 or 561 Yellow PE PI Yellow-Green 561 nm. Red 633 nm. View our complete Brightness Index. Our technical service team is available to help with any questions you may have on multicolor flow cytometry. Technical Service. Phone Toll-Free (US & Canada)1-877-273-3103. Phone (International): 1-858-768-5801. Email: Click Here.Pacific Blue Alexa Fluor 405 LIVE/DEAD Fixable Violet (451) DAPI SYTOX Blue Vybrant DyeCycle Violet FxCycle Violet (DAPI) Annexin V–Pacific Blue Annexin V–eFluor 450 PO-PRO-1 CellTrace Violet Click-iT Plus EdU Pacific Blue Azurite Cerulean TagBFP ECFP mTurquoise AmCyan VioBlue 510/50 520/35 525/50 530/40 550/40 VL2 512/25 VL2 (V6) 525/50 ...

Fluorochrome Peak Channels Laser Center Channel Wavelength (nm) Bandwidth (nm) Wavelength Start (nm) Wavelength End (nm) vs Violet V12 V13 VI 4 428 4/1.3 ... Pacific Blue BV4SO 506 VioGreen 8V570, Pacific Orange BV605,Su ere Qdot605 BV650, Supergright 645, Qdot 655 BV71 su Qdot705 ev786, Qdot goo Blue Excited FluorsSearch worldwide, life-sciences literature Search. Advanced Search Coronavirus articles and preprints Search examples: "breast cancer" Smith J"breast cancer" Smith JTwo of the most commonly used fluorophores in flow cytometry, R-phycoerythrin (R-PE) and allophycocyanin (APC), are phycobiliproteins originally derived from algae. Both proteins contain fluorescent …Fluorophore type. Organic dye. Organic dye. Organic dye (tandem), resonance energy transfer from PE molecule to eFluor 610 dye. Organic dye (tandem), resonance energy transfer from PerCP molecule to eFluor 710 dye. Ex (nm) 475. 546. 496, 565. 488. Em (nm) 535. 585. 607. 710. Application. ICC, IHC. ICC, IHC. FC. FC. Conjugates available : eFluor ...Spectrum [Pacific Blue] Pacific Blue is a fluorescent compound with an excitation peak at 404 nm and an emission peak at 455 nm. Other spectra of interest include: iFluor 405, iFluor 440, and iFluor 430. Pacific Blue belongs to the following categories: Amine Reactive Dyes and Probes for Conjugation. Purchase Pacific Blue here.

Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. We selected 7-hydroxycoumarin as our fluorophore for this study because its excitation and emission wavelengths are suitable for live-cell imaging (excitation 387–405 nm; emission 448 nm) , it is brighter than Enhanced Blue Fluorescent Protein (EBFP) [ε = 36,700 M-1 cm-1 and QY 0.7 for the anionic form , compared to ε = 31,500 M-1 cm-1 and ...

An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter’s ligase ([superscript W37V]LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, [superscript E20G/W37T]LplA, that ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Pacific Green dye is a bright green-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 411/510 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 ...Pacific Blue dye. Fluorescence SpectraViewer. Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. See the characteristics of Pacific Blue dye and the range of antibody conjugates, kits, and other products related to this fluorophore.Pacific Orange and Pacific Blue antibody labeling kits enable attachment of a violet light-excitable fluorescent label on antibodies (up to 100 µg per reaction). Each kit contains …Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells 2011 Sep 27;50 (38):8221-5. doi: …Pacific Orange Dye Cascade Blue and Other Pyrene Derivatives Cascade Yellow and Other Pyridyloxazole Derivatives Naphthalenes, Including Dansyl Chloride Dapoxyl Dye Bimane Data Table Ordering Information Related Tables Molecular Probes amine-reactive dyes—Table 1.1 Active esters and kits for labeling proteins and nucleic acids—Table 1.2Our Fluorophore Equivalency Tool will help you find BioLegend’s equivalent fluorophores compared to other commercially available fluorophores. Simply select the name of a competitor's fluorophore from the dropdown menu to see BioLegend's equivalents. The tool also allows you to preview the excitation and emission spectra of our fluorophores ...Reagent Selection Guide for the Attune Flow Cytometers. The Invitrogen Attune cytometers can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers.

from that of commonly used green fluorophores. Alexa Fluor 405: Excited by 405nm violet laser. Alexa Fluor 405 is an extremely dim dye, for that reason it should be only used for the brightest antigens. It is a derivated of the Cascade Blue dye. Alexa Fluor 405 conjugates show minimal spectral overlap with green fluorophores and

Alexa Fluor 405 dye. Invitrogen Alexa Fluor 405 dye is a blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. Alexa Fluor 405 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.

In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs. antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins. Not for resale.Alexa Fluor 647 dye. Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high ...We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach …Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...Once on the outer surface of the membrane, PS can be detected by fluorophore-labeled annexin V in a calcium-dependent manner. Figure 1 Jurkat cells (human T-cell lymphocytes) untreated control (panel A) or treated with 10 µM camptothecin for 4 hours (panel ... R-phycoerythrin (R-PE), allophycocyanin (APC), and Pacific Blue dye, as well as an ...Shen et al. [85, 94] demonstrated that in rat serum, the conjugated fluorophore (i.e., Alexa Fluor 488) was lost, based on maleimide, yet, ... Structure-guided engineering of a Pacific blue fluorophore ligase for specific protein imaging in living cells.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations ...

The amine-reactive Pacific Blue™ succinimidyl ester can be used to can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ∼410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. View all Pacific Blue™ dye products.. View the Fluorophore Selection Guide. Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Fluorochrome chart FL 450/50 530/30 585/40 630/30 670/40 720/40 780/60 RB Waeeth 3 400 450 500 550 600 650 700 750 800 850 R-PE-Cy7 Cy7 APC-Cy7 AF700 APC-Cy5.5 er DRAQ5 er AF647 R-PE-Cy5.5 R-PE-Cy5 Cy5 APC 7-AAD TR AF594 R-PE-TR PI AF568 R-PE AF555 Cy3 FITC AF488 GFP PB BV421 AF405 DAPI ee uorescet chae n lue chnnel …Instagram:https://instagram. computation of cost of equitywhat time is 6pm eastern time in central timeclas.ujp holley funeral home obituaries columbia sc The BFP filter combination is recommended when studying the following fluorophores: blue fluorescent protein, enhanced blue fluorescent protein, super glow blue fluorescent protein, Cascade Blue, catecholamine, fluorescamine, hydroxycoumarin, Leucophor SF, Pacific Blue, and TNS. The images presented in Figure 2 demonstrate the performance of ...The Fluorophore Brightness Index Score is a relative indication of fluorescence intensity above the background for each fluorophore antibody conjugation (1=dim, 5=brightest). These values can differ depending upon the flow cytometer, the instrument filters and settings, laser power, the antibody clone and the antigen target, f/p ratios, the ... sophia university tokyoscot pollard hair Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. kumed center Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low ...* The excitation laser line represents commonly used lasers that excite the fluorochrome. It does not necessarily reflect the lasers available for each particular instrument. † APC and PE-Cy5 may be used together on instruments with cross-beam compensation. ... Pacific Blue ™ Blue 401 405, 407 ...* The excitation laser line represents commonly used lasers that excite the fluorochrome. It does not necessarily reflect the lasers available for each particular instrument. † APC and PE-Cy5 may be used together on instruments with cross-beam compensation. ... Pacific Blue ™ Blue 401 405, 407 ...