Blue pippin.

Blippi visits the pumpkin playground for kids! Blippi special Halloween videos for Kids. In this educational video for kids Blippi teaches us about Halloween...Data Description Background. Yellowhorn (Xanthoceras sorbifolium) (NCBI: txid99658) is a woody oil species [] that belongs to the Sapindaceae family and the monotypic genus Xanthoceras.As an endemic and economically important species in northern China, it is widely used for soil and water conservation owing to its capacity to survive on arid, saline, and alkaline land and in extreme ...Cassette Kit Product Number Cassette Type Software DNA Control Kit Product Number BLG7510 0.75% and Capture 2 - 5 kb Marker D1 CBC7501 BLG7510 0.75% Band Capture 5 - 10 kb Marker D1 CBC7601It was then centrifuged at 5000 × g for 10 min at 4°. The solution was then purified, washed, and eluted using the Qiagen genome-tip kit instructions. Sequencing libraries were created by shearing DNA to 35 kb on a Megaruptor (Diagenode) and selecting for 18-50 kb using a Blue-Pippin (Blue Pippin system, Sage Science, Beverly, MA, USA).

Postal address. Attn. Janneke Weiss. Genome Technology Center (Route 848) Radboud University Medical Center. Geert Grooteplein Zuid 30. 6525 GA, Nijmegen. The Netherlands. [email protected] Tel: +31 (0)24 3617891 Postal address Attn. Janneke Weiss Genome Technology Center (Route 848) Radboud University Medical Center Gee.

PULSE1: Pippin Pulse Starter Bundle1, with Gel Box, Notebook, and KBB BufferSafety Data Sheet In accordance with Regulation (EC) No 1272/2008 (REACH) Trade name: BluePippin™ Agarose Gel Cassettes Product No: BDQ3010, BEF2010, BDF2010, BDF1510, PAC20KB, PAC30KB,

Page 92 978-922-1832 www.sagescience.com [email protected] 500 Cummings Center Suite 2400 Beverly, MA 01915 25-15 BluePippin Operations Manual 460013 Rev F... View and Download Sage Science BluePippin operation manual online. DNA Size Selection System. Size Select Up To 24 Samples In One Run. PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and ...The Iso-Seq library was constructed using the Clontech SMARTer PCR cDNA Synthesis Kit and the Blue Pippin Size Selection System protocol, according to the Iso form Sequencing protocol (Iso-Seq), as described by Pacific Biosciences (PN100-092-800-03). Oligo magnetic adsorption was used to enrich Poly (A) mRNA. cDNA fragments …BluePippin Operations Manual 460013 Rev K 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an

Then, genomic DNA was fragmented with G-tubes and end-repaired to prepare SMRTbell DNA template libraries (with fragment size of >10 Kb selected by the Blue Pippin system). After the average fragment size was estimated on a Bioanalyzer 2100, SMRT sequencing was performed on the Pacific Biosciences Sequel.

1. Use wide-bore tips for all pipette mixing steps. 2. Always follow Qubit best practices: Prepare the Qubit HS working solution by diluting the Qubit reagent 1:200 in Qubit HS

Technical note ALTERNATIVE SIZE SELECTION METHODS FOR SMRTBELL ® PREP KIT 3.0 Introduction In Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0, size-selection beads are used to remove short DNA fragments and enrich for the long fragments that are most valuable for downstream analysis.Automated, preparative gel electrophoresis systems, used for accurate and reproducible size selection/collection of DNA (or cDNA) fragments, for the preparation of libraries for next generation sequencing (NGS) The supplier does not provide quotations for this product through SelectScience. Browse these related products instead.The ColourTex® stainless steel range is unique whereby the colours and tones of the product varies in different lighting conditions. This is known as a ‘living’ colour when viewed from different angles, also known as metamerism. The ColourTex® process can be combined with the surface finishes of mirror, satin and other polishes, Granex ... After purification of the second PCR product, fragments of a size suitable for sequencing (300–800 bp) are selected using a DNA size selection system (for example, Blue Pippin DNA Size Selection System; Sage Science, USA). 3. The DNA library of size-selected fragments is purified again with AMPureXP Beads.Cells were then counted using trypan blue staining. In total, 6,000 cells per well of a 96-well ultra-low attachment plate (Corning) were seeded. Once the plate was seeded, it was spun down at 234 ...The BluePippin is a DNA size selection system from Sage Science. DNA size selection for next generation sequencing allows for separation from 100 bp - 50 kb using a pulsed-field electrophoresis. Target sizes or ranges entered in the software that elutes fractions in buffer. The software allows the user to run a single sample up to 5 samples/gel cassette with no possibility of cross ...

Pippin Prep DNA Size Selection System Selects 100 bpd - 1.5 kb. SageELF Whole Specimen Fractionation System DNA (up to 40 kb) or Proteins (up toward 500 kDa). Pippin Pulses Electrophoresis Power Supply Resolves DNA up to 400kb on an midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...The Blue Pippin is utilized for DNA size selection (up to 50kb) for NGS. Desired target sizes or ranges of sizes are entered in software and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination....Description. DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field. (100bp - 50kb) Size-Select DNA Fragments up to 50kb Collect HMW DNA with High-Pass Filtering. The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA.Geographic variation in West African Agama picticauda: insights from genetics, morphology and ecology Sneha Krishnana, Caleb Ofori-Boatengb, Matthew K Fujitac and Adam D Leachéd aColorado State University, College of Veterinary Medicine and Biomedical Sciences 1350 Center Ave, Fort Collins, CO 80521, Fort Collins, CO, USA 80526, 4257858934; bKwame Nkrumah University of Science and为了对它们的生态学和进化有更多的了解，我们对三株从抑制病害土壤中分离出来的有很好抑菌效果的链霉菌菌株进行了全基因组测序，并将它们与之前测序的菌株进行比较。. 文章展示了从头测序（de novo sequencing）获得的3个链霉菌菌株的高质量全基因组 ...News and Events 17-04 Reportage NRC handelsblad: 'Is het een schimmel, is het een virus?Sequencers profileren ziektekiemen razendsnel' hierin vertelt o.a. bio-informaticus Jordy Coolen over het sequencen op de apparaten van ons technology center. 22-12 PacBio long-read sequencing in collaboration with the Senckenberg Research Institute and …

Approximately 30 μg of genomic DNA was used to construct PacBio libraries by shearing into ~ 20 kb targeted size fragments with Blue Pippin (Sage Science, Beverly, MA, USA).

Newtown pippin apples 2. Newtown Pippin apple. Newtown Pippin is a popular, all-American green apple from New York City. This apple has a smooth peel, often with a bit of gold russeting on it. The peel is a true green when freshly picked, but matures to a yellow as the apple becomes sweeter in storage.Due to the limited input DNA amount of 1 μg, the library was not additionally size-selected by, for example, Blue Pippin or SAGE Science. Sequencing-by-synthesis was performed on a HiSeq 3000 with 2 × 150 bp paired-end reads at the Max Planck Genome Centre Cologne, Germany.Blue Pippin size selection, high MW DNA selection : $36 per sample: $59 per sample: DNA/RNA quantification. More info about DNA/RNA quantification. DNA/RNA quantification; Description Internal price (Cornell and Cornell affiliates) External price; Digital PCR, Illumina library quantification, reactions set up by the Genomics Facility:Libraries were prepared with Illumina TruSeq Stranded mRNA kit starting from 600 ng of total RNA, and samples were fragmented and amplified for 15 PCR cycles. Libraries were size selected with Blue Pippin (Sage Science) using 1.5% gel cassettes and 350–550 bp regions isolated.The Blue Wizards as seen by Ralph Damiani. The time that the Blue Wizards arrived in Middle-earth is uncertain. In Unfinished Tales, Tolkien wrote that the five Istari came to Middle-earth together in TA 1000. However, in The Peoples of Middle-earth, they are said to have arrived in the Second Age, around the year SA 1600, the time of the ...Individual libraries were normalized to 10 nM and pooled. The library pool was purified with the QIAquick PCR Purification Kit (Qiagen AB, Sweden) according to instructions. Automated size selection was performed using the Blue Pippin (Sage Science, Beverly, MA, USA), with a range of 135 to 175 bp to select the ~152 bp fragments.Bob Pippin is an Allstate Personal Financial Representative from Blue Springs, MO offering Life Insurance, Annuities, ... Bob Pippin, Blue Springs, MO. CLTC. Agent. Bob Pippin, Blue Springs, MO. Contact. Appointment. 707 N 7 Hwy. Blue Springs, MO 64014. US (816) 517-8615 (816) 517-8615. Email.N. crassa OR74A P4C2 SAMN02724975 BashingBeads, Zymo Research kit 6 none Blue Pippin (4kb) N. crassa T1 P4C2 SAMN02724976 SDS, proteinase K, phenol-chloroform, RNAase, isopropanol 15 none Blue ...Blue pumpkins are not widespread commercially, but the pumpkins offer a thick, smooth, and mildly flavored flesh for sweet and savory culinary preparations. Nutritional Value Blue pumpkins are a source of fiber to regulate the digestive tract, and beta-carotene, a natural compound within the flesh converted into vitamin A in the body.Contact for: PacBio, Blue Pippin, Bioruptor [email protected]. Siranoosh Ashtari. Contact for: NovaSeq, NextSeq & MiSeq sequencing, microRNA library preps, and Covaris [email protected]. Emily Kumimoto. Contact for: Illumina sequencing library preparations [email protected]. Ruta Sahasrabudhe, PhD

News and Events 17-04 Reportage NRC handelsblad: 'Is het een schimmel, is het een virus?Sequencers profileren ziektekiemen razendsnel' hierin vertelt o.a. bio-informaticus Jordy Coolen over het sequencen op de apparaten van ons technology center. 22-12 PacBio long-read sequencing in collaboration with the Senckenberg Research Institute and …

Cassettes are disposable and pre-cast with agarose and running buffer. Samples are not optically detected.Blotting kits include all the components required to transfer protein, DNA, or RNA samples onto membranes for staining. The materials are grouped specifically for the method and the tissue specimen being implemented, allowing the entire procedure to be completed more rapidly. Using the ...

Background Orius laevigatus, a minute pirate bug, is a highly effective beneficial predator of crop pests including aphids, spider mites and thrips in integrated pest management (IPM) programmes. No genomic information is currently available for O. laevigatus, as is the case for the majority of beneficial predators which feed on crop pests. In contrast, genomic information for crop pests is ...First, a blue Pippin (SAGE sciences) was used to size libraries before PCR amplification (elution set between 50 and 65 min, on a 2% agarose gel); second, plate barcoding was used to enable sequencing on a shared Illumina NovaSeq 6,000 S4 PE150 lane, as described in Colston-Nepali et al. (2019). The 54 DNA samples were added to a larger pool of ...BluePippin front panel USB Port Indicator Light: Indicator Light: Blue when instrument is Green when electrophoresis is ready for use (power is on, underway (protocol is running). and …For PacBio libraries, we followed the study of Chakraborty et al. by shearing the DNA using a 1.5-inch 24-gauge blunt-tipped needle, and the library was prepared using PacBio's SMRTbell template protocol and Blue Pippin size selection using a 15- to 50-kb cutoff. We generated several dozen libraries in this manner and collected 124 total ...... Blue Hens with 18 points and 18 rebounds (career-best) in the B1G 83-80 win over Illinois on Nov. 26 • Concluded her career totaling 401 points, 367 ...Briefly, genomic DNA was sheared to ~8 KB using g-tubes (Covaris, Woburn, MA, USA) and size-selected with the Blue Pippin system (Sage Science, Beverly, MA, USA), collecting fragments that ranged ...As a new type of fish, GF-L had a high fertilization rate (91.62%) and a hatching rate (82.67%). GF-L presented very beautiful phenotypes, such as twin tails, blue eyes and a white body accompanied by red spots. These phenotypes were very different from any other GF phenotype, showed that the GF-L lineage had great potential in the ornamental ...Blizzard has revealed all the May 2023 Trading Post items and costs, including Pippin the robot-dog pet, the fancy transmog helm Lost Crown of the Arcane, and bonus monthly reward Savage Green Battle Turtle Mount. May 2023 Trading Post Items. ... I hope to see a blue version in the future that matches the Vindictive Gladiator's Chain Armor ...The HLS2 system provides a new approach to DNA extraction. Users load cells suspensions or cell nuclei on Sage Science gel cassettes to perform lysis under electrophoretic conditions. This removes degraded and solubilized proteins but leaves intact DNA behind, bound in agarose. A cleavase is then used to carefully fragment the DNA into ...Size Select Up To 24 Samples In One Run. PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and ...

The ColourTex® stainless steel range is unique whereby the colours and tones of the product varies in different lighting conditions. This is known as a 'living' colour when viewed from different angles, also known as metamerism. The ColourTex® process can be combined with the surface finishes of mirror, satin and other polishes, Granex ...The BluePippin is a preparative electrophoresis platform for collection of size-selected DNA or protein samples. The system automates DNA or protein size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer.The resulting SMRTcell templates were size-selected to 15–50 kb by Blue Pippin electrophoresis (Sage Sciences) and sequenced on a PacBio RS II instrument using P6-C4 sequencing chemistry. Data ...I've found that if I use 3 serial 80% washes, my DNA is retained and I don't have salt problems in the Blue Pippin. ( N.B. I deliberately leave a little excess behind in the first 2 washes to ...Instagram:https://instagram. what time does ucf playcsl plasma busy timesgohcl combest th 11 army Then optimized PCR reactions were used to generate double-stranded cDNA, followed by size selection (fragments larger than 4 kb) using a Blue Pippin TM Size-Selection System (Sage Science, MA, United States) as described by PacBio (PN 100-092-800-03). The generated cDNA was then re-amplified using PCR to obtain sufficient cDNA.SDS-GHS: Reagent Replacement Kit for Dye Free Pippin Prep Cassettes (RIP2010, RIK2010, RIK 1510) SDS-GHS: Pippin Prep Cassettes with Ethidium Homodimer, Reagent Replacement Kit (REK2010) SDS-GHS: 10X KBB Electrophoresis Buffer (KBB1001) SDS-GHS 0.5X KBB buffer (PN190039) SDS-GHS: DNA Control Kits for Pippin Prep and BluePippin. gonzaga vs kansasgacha life body clothes Fragments smaller than 20 kbp were removed using the Blue Pippin Size selection system (Sage Science, Beverly, MA, USA) and the constructed libraries were validated using Agilent 2100 Bioanalyzer ... craigslist houses for rent emmett idaho Upgrading Pippin Instrument Software 1. Insert the USB drive with the upgrade folder into the USB port on the front panel of the BluePippin instrument. 2. From the Main Tab, press the “INFO” button. This will launch the information window. 3. From the Information Window, press “SOFTWARE UPGRADE”, press“OK” at the warning prompt ... BluePippin Operations Manual 460013 Rev B 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.Size selection was performed at the University of Maryland Genomics Resource Center using a Blue Pippin pulse-field gel electrophoresis instrument. A library was constructed and 24 SMRT cells were sequenced on their PacBio RS II using the P5-C3 chemistry. ... The bottom (blue) track shows the testis transcriptome assembly aligned to M_zebra ...