Blue pippin.

手工切胶回收Pippin Prep/Blue Pippin 几个小时的手工操作 几分钟的手工操作 回收的核酸片段大小不精确 精确回收指定大小范围的核酸片段 存在小分子量（LMW）污染 去除小分子量（LMW）污染 30-50％的回收效率 50-80％的回收效率 回收中、小核酸片段 可以回收大的 …Library preparation and sequencing. We prepared three pooled sequencing libraries for the 438 samples following the 3RADseq protocol as described by Graham et al. (), with one modification: we size-selected for 650-850-bp fragments on a Blue Pippin (Sage Science).The concentration of each index group was determined by using a Qubit, and the average fragment length was determined by using a ...Biscotti is a rare indica dominant hybrid strain (80% indica/20% sativa) created through a delicious cross of the classic Gelato #25 X Girl Scout Cookies X South Florida OG strains. If you're looking for a classic indica with an insanely delicious flavor, you've found it. Biscotti packs everything you want and more in the effects and taste ...Based on the results, we combined pools at equimolar concentrations and size selected for a range of 250-600 bp with a Blue Pippin device (Sage Science, Inc.). In the other laboratory (laboratory 2), we measured DNA quantity with Qubit, examined fragment distributions with TapeStation, and then size-selected pools showing excessive large ...

For Nanopore sequencing, high-quality genomic DNA with a large fraction was selected using Blue Pippin (Sage Science, USA), followed by end-repair/dA tailing. End-repaired DNA fragments are processed for adapter ligation using a ligation sequencing kit (NBD103 and NBD114, Oxford Nanopore Technologies USA). Finally, the DNA library was ...See full list on sagescience.com The complementary DNA (cDNA) was synthesized using the Clontech SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA). Fragments >4 kb were selected using the Blue Pippin Size Selection System protocol (Sage Science, Beverly, MA, USA). The >4 kb cDNA and normal cDNAs were mixed in equimolar amounts for SMRT sequencing.

Individuals were then pooled, followed by a size selection step with the Blue Pippin (Sage Science, Beverly, MA) where all loci between 415 and 515 bp were retained. A final PCR step using Phusion Taq polymerase (New England Biolabs) with the following thermocycler conditions was conducted to amplify all loci and attach a 6bp index unique to ...

Blue Pippin Size-Selection System. The first step of the protocol was cDNA synthesis with adapters. and PCR cycle optimization followed by large-scale PCR amplification. PCR products were then size.Upgrading Pippin Instrument Software 1. Insert the USB drive with the upgrade folder into the USB port on the front panel of the BluePippin instrument. 2. From the Main Tab, press the "INFO" button. This will launch the information window. 3. From the Information Window, press "SOFTWARE UPGRADE", press"OK" at the warning prompt.The Blue Pippin is a powerful automated tool to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular Next Generation Sequencing platforms. Features : Saves time and effort compared to manual gel purification; Sample-to-sample reproducibility streamlines researchducted using the Blue Pippin automatic nucleic acid fragment recovery system. Then, barcode was added by a PCR-free method using the EXP-NBD104 kit (Oxford Nanopore Technologies Company). Afterward, the size of fragments was detected by the AATI auto-matic capillary electrophoresis instrument, and the samples were isomolar mixed. Finally, the

The BluePippin produces higher quality DNA, improving downstream analysis. Pre-cast agarose gel cassettes are constructed in a way that eliminates cross contamination. WHY use the BluePippin? (Advantages) The CGRB houses multiple Illumina and Pacific Biosciences next generation sequencing platforms that all have applications that benefit from ...

The importance of structural variants (SVs) for human phenotypes and diseases is now recognized. Although a variety of SV detection platforms and strategies that vary in sensitivity and specificity have been developed, few benchmarking procedures are available to confidently assess their performances in biological and clinical research.

GeneScan 500 ROX dye Size Standard, each. $2.25 (minimum fee $50) GeneScan 500 LIZ dye Size Standard, each. $2.25 (minimum fee $50) GeneScan 400HD ROX dye Size Standard, each. $2.25 (minimum fee $50) MapMarker X-Rhodamine Labeled 50-1000bp, each. $1.80 (minimum fee $50) GeneScan 1200 LIZ dye Size Standard, each.Fragments smaller than 20 kbp were removed using the Blue Pippin Size selection system (Sage Science, Beverly, MA, USA) and the constructed libraries were validated using Agilent 2100 Bioanalyzer ...(B) The final indexed PCR products were purified using Ampure XP beads using BlueCat BlueWasher, pooled into a single library using Hamilton Starlet, and size selected using Sage Science Blue Pippin.Upgrading Pippin Instrument Software 1. Insert the USB drive with the upgrade folder into the USB port on the front panel of the BluePippin instrument. 2. From the Main Tab, press the “INFO” button. This will launch the information window. 3. From the Information Window, press “SOFTWARE UPGRADE”, press“OK” at the warning prompt ...Like the Blue Pippin, the PippinHT has pulsed field capabilities, thus enabling the size selection of DNA fragments intended for use in both short-read (including the Illumina iSeq 100, MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, Ion Torrent PYRO 454) and long-read (Oxford Nanopore and PACBIO) NGS workflows. Proprietary Electro-elution Technology

The ~500 bp insert size libraries were sequenced on Illumina HiSeq 2500 (2 × 125 PE). The 3 DNA candidates were also sent for PacBio library construction. To do this, 10 μg gDNA was sheared to an ~20-kb targeted size using a Covaris g-Tube, then used for size selection using Blue Pippin (Sage Science, Beverly, MA, USA).Amplified cDNA was fractionated into > 4 kb fragments by Blue Pippin Size Selection v5.90 (Sage Science, Beverly, MA, USA) and subsequently used to construct one library of different insert sizes ...The Blue Pippin facilitates library construction for most popular NGS platforms, and is recommended by Pacific Biosciences, Illumina and Ion Torrent for certain workflows. Utilizing pulsed-field electrophoresis it is an ideal solution for resolving and collecting high molecular weight DNA up to 50kb. The platform features the ability to collect ...Air Pippen. $665. Kith x Air Pippen 1 'Friends & Family'. 2017. $1,007. Air Pippen. Shop Air Pippen 1 shoes on GOAT. Featuring new, upcoming and iconic styles including the Air Pippen 'Black Varsity Red' 2008, Air Pippen, Air Pippen 'Black Varsity Red' 2008 and more. Buyer protection guaranteed on all purchases.Posted on August 3, 2023 by Alex. Range+T Size Selection Sage’s DNA size selection technology has been used to help improve PacBio sequencing, particularly for mammalian and complex genome work since the introduction of the BluePippin (2012). Over the last few years, PacBio®’s Hi-Fi circular consensus sequencing … more ».The blue-rumped parrot (Psittinus cyanurus) is a parrot found in the very southern tip of Myanmar, peninsular Thailand, Malaysia, Borneo, Sumatra and nearby ...The resulting SMRTcell templates were size-selected to 15–50 kb by Blue Pippin electrophoresis (Sage Sciences) and sequenced on a PacBio RS II instrument using P6-C4 sequencing chemistry. Data ...

The core facility staff will wear face mask in the laboratory, which hosts multiple instruments for shared use by researchers via scheduling. Users are required to follow the University's COVID-19 Policies and Guidance on this web page: The primary contact for the Genomics Core Facility is Wei Wang, the Director, [email protected], 609-258-6998.

The entire elution was size selected on a 3% agarose cassette using the Blue Pippin system, targeting either 190 or 200 bp using "tight" settings. The 40 μL elution was collected from blue pippin and analyzed using High Sensitivity DNA bioanalyzer. Dilution is usually not necessary for this pool, but think about your expected concentration ...The library preparation method is similar to the Ligation Sequencing Kit protocol; DNA ends are repaired and dA-tailed using the NEBNext End Repair/dA-tailing module, and then a unique dT-tailed barcode adapter is ligated on the dA-tailed template.Product Type. Agarose Gel Cassette. Separation Range. 100 to 600 bp. Quantity. 10 Pack. Provide Content Correction. For size selections between 100bp and 600bp. Sage Science™ BluePippin™ 2% Agarose Gel Cassettes are commonly used for Illumina paired-end library construction, Ion Torrent template prep, and ChIP applications.Are you looking for health insurance? Blue Cross insurance is one provider option that is widely available and, therefore, is likely to come up in your search. Learn more about where and how Blue Cross operates, including information on wha...Pippin: What about breakfast? Merry: You've already had it. Pippin: We've had one, yes. What about second breakfast? Merry: Don't think he knows about second breakfast, Pip. Pippin: What about elevenses? Luncheon? Afternoon tea? Dinner? Supper? He knows about them, doesn't he? Merry: I wouldn't count on it.BluePippin™ DNA Size Selection Instrument. Client: Sage Science. Tags: DNA Extraction & Purification, Instrumentation Life Sciences, NGS. The BluePippin is a preparative …The BluePippin produces higher quality DNA, improving downstream analysis. Pre-cast agarose gel cassettes are constructed in a way that eliminates cross contamination. WHY use the BluePippin? (Advantages) The CGRB houses multiple Illumina and Pacific Biosciences next generation sequencing platforms that all have applications that benefit from ...Pippin Gel Cassettes. Gel Cassettes are pre-cast with agarose, and use ethidium bromide to visualize DNA and provide more uniform migration of fragments. Cassettes have a capacity to run 4 samples and are shipped in boxes of 10 each. Loading dye and DNA marker (for calibration) sufficient for all 40 runs are provided with each shipment.

Blue chip shares are investments in well-established companies and leaders in their respective industries. Blue chip companies in general are financially stable with a history of solid earnings. Blue chip shares are investments in well-esta...

This instructional video demonstrates the optical calibration procedure for Pippin Prep and BluePippin automated DNA size selection instruments from Sage Sci...

Blue Pippin Instrument Pulsed-field automated DNA size selection system The Sage Science product portfolio combines pre-cast agarose cassettes with a high-precision, automated solution for the accurate and targeted size-selection of DNA fragments for library construction in NGS workflows FlexibilityThe Pippin platform is typically used to construct genomic libraries for next-gen sequencing. The BluePippin is an updated version of Sage's Pippin Prep system, to which a pulsed-field power ...BluePippin Prep. The Pippin Prep is an electrophoresis platform capable of size-selecting DNA fragments and automatically collects the fractions in buffer according to the user-specified size range. It is routinely used to size-select next generation sequencing libraries. It is capable of performing narrow size selections from 50bp to 50kb in ...When it comes to choosing a web host, there are many options available. One of the most popular choices is Blue Hosting. But how does it compare to other web hosts? In this article, we will explore the features and benefits of Blue Hosting ...Product Overview. Documents. GlycoBlue Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant. The attached dye increases visibility of the pellet. This product is an ideal coprecipitant in nuclease protection assays at 1/100 dilution of stock solution.He was an eight-time member of the NBA All-Defensive First Team (1992-1999) and the 1994 All-Star MVP. Yet, his career averages of 16.1 points, 6.4 rebounds and 5.2 assists were not staggering ...The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions. The system is equipped with pulsed-field electrophoresis for resolving large DNA. Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer.Blue pippin size selection elution volume 07-03-2015, 02:20 AM. Hello, Has anyone has tried eluting their TruSeq DNA libraries in a smaller volume than the 40uL stated in the protocol? We carry out size selection on our TruSeq nano libraries after the PCR enrichment step which works for us but gives very low concentrations. I was wondering if ...Pippin Pulse Electrophoresis Power Supply Pulsed-field Power for Gel Boxes. Sage Science is pleased to introduce the new Pippin Pulse electrophoresis power supply for analytical gels. They've taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond ...

The nGBS libraries were then size selected on Blue Pippin, discarding fragments smaller than 300 bp and larger than 500 bp long. Sequencing was carried out on an Illumina NextSeq 500/550 using V2 Chemistry and 300 cycles. Sequences were demultiplexed with Illumina's CASAVA data analysis software and proprietary software developed by LGC Genomics.Size-selection of large fragments on the Sage Science Blue Pippin is available separately or bundled as part of certain library prep packages. Multiplexed library builds are available. The options for this are: barcodes internal to your amplicon(s) (you would have to add these during amplification using PacBio's Barcoded Universal Primers) or ...8 thg 10, 2023 ... Blue, Yellow, Magenta, Cyan. Transparency. Opaque, Semi-Transparent. Background. Color. Black, White, Red, Green, Blue, Yellow, Magenta, Cyan.Instagram:https://instagram. permanent curly hair salon near meexempt from 2023 withholding meaningqualitative vs quantitative assessmentpipe wrench restoration Pippin/BluePippin TYPE: WALKUP or ASSISTED assisted service available as part of library preparation UNIT: Per run for walkup Per lane for assisted FORMAT: 5 lane gel from 0.75% agarose to 3% agarose INPUT >1ug of DNA in low salt buffer EXPECTED YIELD: 10-50% of material in the selected size range Narrower ranges result in lower yield. WALKUP ...Blue Pippin ™ Size Selection System (Sage Science, Beverly, MA) was used for the size selection of different sizes of cDNA libraries (1–2, 2–3, and > 3 kb). According to the instructions, three SMRT libraries were constructed using SMRTbell template prep kit 1.0 (Pacific Biosciences) and were sequenced through seven SMRT … big 12 baseball scores 2023mongoose bike 24 inch BluePippin Operations Manual 460013 Rev E 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an Background. Orius laevigatus, a minute pirate bug, is a highly effective beneficial predator of crop pests including aphids, spider mites and thrips in integrated pest management (IPM) programmes.No genomic information is currently available for O. laevigatus, as is the case for the majority of beneficial predators which feed on crop pests.In contrast, genomic information for crop pests is far ... 10am pdt to central time Blue Pippin was used for fragment size selection between 600 and 1200 bp on a 1.5% agarose gel dye-free cassette (Sage Science). Eluant was purified with 1.8× Agencourt AMPure XP bead system (Agencourt) before analysis with a Bioanalyzer High Sensitivity DNA Chip (Agilent Technologies) and Qubit High Sensitivity dsDNA assay (Life TechnologiesSage science全自动核酸电泳和片段回收系统Pippin，科技视频免费在线观看，视频简介：Sage science全自动核酸电泳和片段回收系统 Pippin Prep and Blue Pippin 首页 电视剧 电影 云影院 综艺 儿童 动漫 游戏 会员中心 纪录片 体育 知识 直播 小说 漫画 风云榜 新片 公益 VR 随 ...3.0 BLUE-PIPPIN INSTALLATION PROCEDURE 3.1 Packaging and Components The system is shipped in two boxes: one will contain the BluePippin instrument and accessories, and the second box will contain the computer monitor in the manufacturer's original packaging. With the boxes in the upright position, open and confirm that the following items are ...